合作类型：技术     行业类别: 医药研发    过期时间：2017年02月27日    

     一家意大利的中小型企业提供心血管药物和药物动力学的体内外模型（包括内皮功能紊乱，炎症，血管再生相关疾病）的创新低成本的临床研究服务。同样该服务还提供生物化学和动力学服务。该企业正在寻找有兴趣外包其临床药理项目，以及联合经营或技术合作的合作伙伴。

英语原文：

Summary:

An Italian SME offers innovative and cost-effective preclinical R&D services focusing on cardiovascular drug efficacy and pharmacokinetics in wide platform of in vitro and in vivo models, including endothelial dysfunction, inflammation and angiogenesis related disorders. Biochemical and reactivity-kinetics services are available as well. The SME is looking for partners interested in out-sourcing their preclinical pharmacology projects, for joint venture or technical cooperation.

Details:

An Italian SME is offering innovative and cost-effective preclinical R&D services:

CHEMICAL AND BIOCHEMICAL SERVICES

Expertise and equipment for the characterization of individual compounds and mixtures of compounds with spectroscopic and chromatographic methods. The following specific methods are available:

- Separation and analysis of mixtures of compounds by HPLC-MS/MS for the identification of individual components

- Purification of the components of mixtures by preparative HPLC

Analysis of compounds, or components of mixtures, and structure determination through the following types of analytical tools:

- Multinuclear NMR spectra (1H, 13C, 31P, etc.)

- Multidimensional NMR spectra (COSY, TOCSY, heterocorrelated spectra, etc.)

- MS spectra with analysis of fragmentation pathways

- IR spectra with analysis of functional group frequencies

- Circular dichroism with analysis of optical activity

-> Reactivity, Enzymatic and Kinetic Studies:

Expertise for the investigation of chemical and chemico-pharmaceutical processes, chemical and biochemical catalysis, innovation and optimization of processes, and characterization of their mechanisms of action. The following specific methods are available:

- Reactivity studies of compounds and analysis of reaction mechanisms, also using fast kinetic techniques (stopped-flow)

- Enzymatic assays, assessment of efficiency and kinetic parameters of catalytic and enzymatic reactions

- Spectroscopic detection of reaction intermediates

- Reactivity studies at controlled temperatures (from -90 °C to +90 °C) and under anaerobic conditions

PLATFORM of PRECLINICAL PHARMACOLOGICAL SERVICES

-> Cellular Biology:

- Isolation of endothelial and smooth muscle cells form human specimen and animal tissues/organs

- Toxicological tests and pharmacological screening of synthesis or biotechnological compounds, and biomaterials

- Functional tests: adhesion, migration and invasion, proliferation and survival, DNA synthesis, apoptosis and senescence

- In vitro angiogenesis tests, evaluation of endothelial functions

Co-culture systems and permeability

-> Molecular Biology and Biochemistry:

- Study of the mechanisms of action through the evaluation of the expression/activation of receptors, signaling systems and enzymatic cascades, production and organization of cytoskeleton proteins and extracellular matrix on cell cultures and tissue sections,

- Validation of signaling through silencing techniques (siRNA) and gene transfect ion (plasmids) on cell culture,

- Evaluation of gene expression through RT-PCR on cell culture and biological samples

- Evaluation of mitochondrial functions and ROS processing

- Measurement of nitric oxide synthase activity, and cGMP accumulation

- Measurement of prostanoids, growth factor and angiogenesis modulators.

-> Ex vivo studies:

- Cardiovascular pharmacology: evaluation of vascular tone in rat aortic rings

- Platelet aggregation

- Angiogenesis test: sprouting formation from mouse aortic ring in 3D gel.

-> In vivo studies:

- Tumor growth in immunodeficient and normal mice (drug local treatment, i.p., gavage), metastasis assay

- Angiogenesis test (Matrigel plug assay in mouse, corneal pocket assay in rabbit)

- Measurement of blood pressure and ECG in conscious animals by tail cuff technique

- Animal maintenance and systemic treatment (acute and chronic drug administration)

- Histological analysis:

Histological analysis in cells and tissues

Expression and localization of proteins by immunohistochemistry and immunofluorescence

Quantification of tumor angiogenesis

The company is looking for partners that are interested in out-sourcing their preclinical pharmacology projects. The company is open for joint venture or technical cooperation.

Type and Role of Partners Sought:

- Type of partner sought: Industry

- Specific area of activity of the partner: Biotechnology, Pharmaceuticals, R&D in cardiovascular disease treatment

- Task to be performed by the partner sought: further clinical co-development of molecules/devices.